Effects of Vanadyl Sulfate on Media Consumption by Satureja khuzistanica Cells and Rosmarinic Acid Biosynthesis

Application of biotechnology for production and enhancing of important secondary metabolite by plant cell cultures and elicitors is one of the most noteworthy researches by scientists. This research was carried out with the aim of analysis the effects of Vanadyl Sulfate (abiotic elicitor) on media consumption by Satureja khuzistanica cells under suspension culture. Cells obtained after four subcultures from suspension culture of Satureja khuzistanica used in the present study. B5 liquid medium containing 5 mg.l-1 BA (benzyl adenine) and 1 mg.l-1 IBA (Indole-3-butyric acid) were prepared. Three levels of Vanadyl Sulfate (0, 2.5 and 5 mg.l-1) were analyzed. According to results, the highest consuming rate of culture media and the lowest cell dry weight obtained by 2.5 mg.l-1 Vanadyl sulfate. Furthermore, concentration of 5 mg.l-1 showed approximately equal content of dry biomass compared to the control condition. The maximum biosynthesis of rosmarinic acid was obtained by cells in media containing 2.5 mg.l-1 Vanadyl sulfate. These results showed that application of Vanadyl sulfate have positive effects on biosynthesis of rosmarinic acid. Altogether application of different concentrations of Vanadyl sulfate as an abiotic elicitor in Satureja khuzistanica showed a normal dry matter production; while rosmarinic acid was increased. Therefore, application of this elicitor in suspension culture of Satureja khuzistanica is suggested to increase rosmarinic acid production.