Endoplasmic reticulum stress regulates inflammation in adipocyte of obese rats via toll-like receptors 4 signaling

To explore whether endoplasmic reticulum (ER) stress regulates inflammation in adipose tissue of obese rats via TLR4 signaling.
Sprague Dawley rats were randomly divided into four groups, and body weight, food intake, and free fatty acids (FFA) were measured. Real-time PCR and Western blot were used to determine mRNA or protein expression of TLR4, TRAF6, IKKβ, TNF-α, IL-6, and GRP78. Immunohistochemistry was used to detect GRP78 protein expression.
The FFA levels in HFD, HFD㰐, and HFD咃 groups were higher than that in the control group (P HFD could induce inflammation in adipose tissue via ER stress and its downstream TLR4 signaling.