The phenotypic and genotypic study of Staphylococcus aureus biofilm genes isolated from clinical and food cases by microtiterpolytte and Multiplex PCR

The most important factor in the formation of Staphylococcus aureus is the ability to form biofilms. This bacterium has the ability to bind and penetrate into tissue cells. Biofilmic infections are usually chronic and reversible and respond to difficult treatment. The purpose of this study was to identify biofilm genes in Staphylococcus aureus isolated from clinical and food samples using multiple PCR methods.
In this study, 60 isolates of Staphylococcus aureus cultured from human and food after extraction of genome were used to study the presence of adherent genes using multiple PCR techniques. The phenotypic study of biofilm formation was performed using Microtiter plates assay microplate technique.
In total, 60 samples of food and humans studied included 6 samples of foodstuff, 20% of samples and of 2 samples clinical 6.67% isolated from biofilms. In 30 isolates isolated from food, the frequency of fnbB, clfB, clfA genes was identified as 20%, 76.66%, 80%, respectively. However, among the 30 clinical samples, fnbB, clfB, clfA genes were 6.66%, 70%, 93.33%, respectively. The fnbA gene was not detected in any of the samples.
The results of the study showed that the high prevalence of biofilm-inducing genes and also a relatively high phenotypic expression among the samples showed that it could be related to the resistance of the isolates of Staphylococcus aureus.