RNA-Sequencing, integrated networking, myogenesis

RNA-Sequencing technique is a powerful tool for analysis of cellular transcriptome in many research areas. In recent years, network constructing on data resulted from this technique and gene relationships have been investigated for better understanding the complex physiological mechanisms. In this study, construction of gene regulatory network and surveying gene relationships which involve in skeletal muscle synthesis were discussed by growing muscle tissue’s RNA-Seq data. This research introduced six transcription factors which were involved in formation and growth of skeletal muscle tissue by fitting an integrated network. These transcription factors included KLF4, FOXM1, FOS, MYBL2, KLF10 and FOSB. Their biological roles have been identified in proliferation and differentiation of stem cells, elongation and fusion of muscle cells, myofibril generation and self-renewal of satellite cells. Ontology of these transcription factors and their regulated genes showed that theyare involved in hexose pathway and nucleotides production. Theyare also involved in regulation of basic cell activities such as gene expression and transcription. These genes may be used as molecular markersfor improvement of meat production in breeding programs.