Phylogenic groups of E. coli isolated from septicemic Japanese quail in Ahvaz

Suitable hygiene keeps low mortality rates in all quail growth stages. One of the most important causes of death in newborns is bacterial infections. Escherichia coli is one of the most important microbial agents in septicemic Japanese quail chicks. It has a global distribution and is part of the natural flora of the digestive system in human and animals.Phylogenetic study indicated that the great majority of E. coli strains can be classified into one of the four phylo-groups (A, B1, B2, and D). This study was conducted to identify the phylogenic groups of E. coli isolated from septicemic Japanese quail in Ahvaz based on the presence or absence of three genes (chuA, yjaA and TspE4.C2). For this purpose, the DNA of 29 isolates of E. coli was extracted and tree mentioned genes were detected by multiplex polymerase chain reaction (Multiplex PCR). The isolates belonged to four main phylo-groups, including 34.48% isolates to A, 17.27%to B1, 10.35% B2 and 27.58% to D. The highest frequency was observed in the phylogenetic group A.