Identification of mutation in tow candidate genes with resistance potential against avian influenza and salmonellosis in some Iranian indigenous and commercial chicken strains

In this study, allelic polymorphism in candidate genes of Mx and SLC11A1 involved in the immune system in some Iranian Common, West Azarbaijani, Marandi, Mazandarani indigenous and commercial chicken strains examined using PCR-RFLP technique. A total of 300 birds were selected and for detection of mutation in Mx and SLC11A1 genes the PCR products were digested by Hyp81 and SacI restriction enzymes, respectively. For the Mx gene, one fragment with length of 299 bp and two fragments with the length of 200 and 99 bp were identified for the A and G alleles respectively. One fragment with the length of 801 bp for T allele and two fragments with the length of 722 and 79 bp for C allele were identified in SLC11A1 gene. Between different groups of chickens, in the Mx and SLC11A1 genes, the most frequency of susceptible to disease alleles (G and C) were in the indigenous chicken strains and meat commercial chicken strain. In the Mx loci, Shannon's information index was in different groups of chicken from 0.1347 to 0.6641 and in the SLC11A1 loci was from 0.3669 to 0.6769. In the Mx loci, fixation index was positive for indigenous chickens group and in the SLC11A1 loci was negative for different groups of chickens. The results of this study indicate that these genes loci can be used as marker for genetic breeding to reduce the diseases of indigenous and commercial chicken strains.