Investigation of the preparationof lysozyme and nattokinase enzymes in chitinous shells media of Litopenaeus vannamei and Portunus pelagicus

In this research, production of lysozyme and nattokinase enzymes by selected bacteria in chitinous shells culture media of Litopenaeus vannamei and Portunus pelagicus were evaluated. Shrimp specimens were collected from fish market centers in Khorramshahr. Crab sampling was undertaken from intertidal areas of Khuzestan coasts, Persian Gulf. For lysozyme enzyme activity, colorimetric assay with ethylene glycol chitin and lysoplate method with M. luteus as substrate were applied. To evaluate nattokinase production, human blood clot lyses method was used. Results showed that unit of lysozyme activity for P. aeroginosa with lysoplate method was 6.47μg/ml, colorimetric method was 35.2 U/ml and NAG released by enzyme activity was 0.091 mg/ml, respectively. Average unit of nattokinase activity for B. subtilis was also %37.4. Therefore, in addition to successful removal of chitinous wastes from environment and extraction of pure chitin, it is possible to manufacture lysozyme and nattokinase enzymes with selected bacteria. These enzymes can be used in medical and pharmaceutical applications after industrial optimization.