Effect of Single Bout of Continuous Exercise and High Intensity Interval Exercise on HIF-1α and TNF -α Levels in Adipose Tissue of Obese Male Wistar Rats

The purpose of this study was to determine the effect of single bout of continuous exercise and high intensity interval exercise on Hypoxia Inducible Factor-1alph (HIF-1α) and Tumor Necrosis Factor-alph (TNFα) levels in adipose tissue of obese male Wistar rats. Subject was 30 obese male Wistar rat that intake high fat diet for 8 weeks. Subject done an exhaustive test to assess VO2max for determine the exercise intensity then divided into three groups: Control, continuous and High Intensity Interval Exercise (HIIE). The HIIE protocol was five 2min sets of high intensity exercise (100% vVO2max) which separated whit four sets of low intensity exercise (30% vVO2max). Continues group intensity was 65% vVO2max and the running time measured by interval sets distance in HIE group. Western blotting analysis use for determine protein levels in visceral and subcutaneous adipose tissue and data analysis was performed with SPSS software. Based on data HIF-1α level reduce significantly 33.5% and 45% in continues and HIIE group respectively (P