Investigation of antioxidant, anti-bacterial properties and binding to human serum albumin in the Cressa cretica L. grown in the Sistan region

Cressa cretica L., due to producing of some various compounds, which could be used as a source of many herbal medicines. The present research were studied in antioxidant, antibacterial properties and the ability of interaction with human serum albumin (HSA) of Cressa cretica L. So the leaves of the plant were collected in vegetative stage from Sistan in 2015. Three kinds of extraction such as aquatic, methanolic and ethanolic extracts were obtained by using maceration method. The antioxidant activity were measured by DPPH method. The interaction of ethanolic extraction with HSA was performed by UV-Vis spectroscopy. The Anti-bacterial properties of extracts against Staphylococcus aureus, and Escherichia coli were investigated by MIC and MBC methods. The results were showed that the lowest and highest extraction efficiency were related to the aquatic and ethanolic, respectively. The ethanolic extract had the highest antioxidant activity in inhibition percentage of DPPH than other extracts (56, 38 and 34%) respectively. Study of the interaction with HSA demonstrated the changes in protein secondary structure and the binding constant in that obtained 0.49 × 102 ppm-1.This plant showed acceptable bactericidal activity and MIC values were higher than the MBC.