The effect of creatine supplementation on the activity of creatine kinase and lactate dehydrogenase enzymes after an intense exercise session in athletic girls

Creatine is one of the most important and commonly used supplements among athletes, although the molecular mechanisms by which it exerts its effect and its side effects are less understood. The aim of this study was to determine the effect of creatine monohydrate supplementation on the activity of creatine kinase and dehydrogenase enzymes after an intense exercise test in athletic girls.
After obtaining informed written consents, 30 trained girl students were selected through 7-stage Bruce Test to participate in this study and were randomly assigned to experimental (creatine supplementation / 7 days, N = 15) and control (placebo, N = 15) groups. The blood samples were collected before supplementation (pre-test) and 24 hours after Cunningham Test (post-test) to measure the levels of creatine kinase (CK) and lactate Dehydrogenase (LDH) in the two groups.
There was no significant difference in the levels of CK (p = 0.621) and LDH (p = 0.852) between the two groups at baseline (pre-test). No significant difference was observed between pre- and post-test levels of CK (p = 0.214) and LDH (p = 0.325) in the experimental group. All the variables did not change in the control group (p > 0.05).
Based on these findings, creatine monohydrate supplementation did not have a significant effect on the activity of creatine kinase and dehydrogenase enzymes after an intense exercise test in athletic girls.