Effect of juvenile tissue and plant growth regulators on in vitro shoot proliferation of Pronus avium L

Propagation of Pronus avium L. through conventional micropropagation is essential for forest development and production of scion for grafting of commercial Pronus varieties. For micropropagation and production of juvenile tissue as an initial source of proliferation, mature embryo explants were used. For in vitro shoots preparation, isolated embryos of mature trees were transferred into MS medium containing 30 and 60 g/l sucrose. There were no significant differences between MS media containing 30 and 60g/l for percentage of embryo germination using t-Student test. Factorial analysis of collected data for further shoot proliferation indicated that there were highly significant differences between the used growth regulators for in vitro shoot length and number of produced explantsýatýα=0.01ýlevel. The highest average shoot length (5.86 cm) and the highest mean number of explants production were observed on MS medium supplemented with 4 mg/l BA and 0.2 mg/l IBA. Plantlet at 4 to 5 cm in height were rooted in MS hormone free medium and after successful acclimatization transferred to a green house.