Asexual regeneration of (Zhumeria majdae L.) by Bud culture

Zhumeria majdae is one of the most important aromatic plant species from Lamiaceae family. The valuable species has medicinal and industrial applications. It is an endemic and endangered species of Iran. Therefore, tissue culture techniques are proposed for propagation and conservation purposes of the species. Usual regeneration of the species is by seed. Apical buds of seedlings from Hormozgan genotypes were sterilized by HgCl2 0.1% solution for 5 minutes during spring season. The best medium for shoot proliferation was WPM containing BA (0.5 mg/l), 2iP (0.3) and IBA (0.1 mg/l). The highest rooting of the shoots was obtained by WPM supplemented with 1 mg/l of IBA. The plants were acclimatized in greenhouse after transferring in soil. The protocol of this research could be used for culture and developmental programs of the species in its original habitat.