Repair ethanol induced amnesia with lactobacillus rhamnosus, Iranian probiotic, in male wistar rats

Probiotics are food supplements and pharmaceutical substances prepared from living microorganisms that with enhancement of microbial equilibrium of intestinal microbiota, insert useful effects on the host (human/animal). Nutrients could affect the brain and neurohormones via vagus nerve. Therefore, probiotics can regulate some parts of brain activities and effect in better function. The aim of this study was examination of Lactobacillus rhamnosus, the native Iranian probiotic isolated from Tarkhineh dough (a carbonated yoghurt drink) product on the rat passive avoidance. We used 56 male Wistar rats with 250-300 gr weight range. For induced amnesia intra peritoneal pre-training injection ethanol and for one mount administrated with1×10 9 CFU/ml of with or without probiotic bacteria. We used the step-through passive avoidance to examine long-term memory in male Wistar rats. Post-training subcortical (SC) administration of ethanol (1 mg/kg) induced amnesia. This amnesia was reversed in probiotic receiving groups. L. rhamnosus isolated from Tarkhineh dough, enhanced the passive avoidance learning of male Wistar rats that received ethanol. Our data demonstrated the positive effect of this probiotic on learning and memory.