Primary Culture of Follicular Cells and Oocyte in Sterlet Sturgeon Acipenser ruthenus a Suitable Method to Functional Studies

Primary tissue culture, its applications and using this method for propagating of oocyte follicular cells were explained. Material and methods for isolating and culturing of follicular cells from Sterlet sturgeon, Acipenser ruthenus in L-15 culture medium that complemented with FBS and antibiotics, evaluation of their propagation and steroids hormones secretion expressed. According to obtained results, cultivated follicular cells could be propagated and secret Testosterone (T), Estradiol-17&beta (E2) and Progesterone (P4) hormones in culture medium. In attention to obtained consequences from culturing of follicular cells in Sterlet sturgeon, Common carp, Cyprinus carpio, European sea bass, Dicentrarchus labra and primary culture of oocytes in some fish such as Gold fish, Carassius auratus Medaka, Oryzias latipes Guppy, Poecilia reticulate Fathead Minnow, Pimephales promelas, Persian sturgeon, Acipenser persicus, and a hybrid sturgeon, Bester, developing similar in vitro systems for ovarian follicular cells and oocytes in other fishes that will be useful to functional studies and serves a valuable tool in study on oocytes and follicular cells.