Identification and Quantification of Gliclazide in Human Plasma by HPLC-PDA

In this study, a high-performance liquid chromatography method was developed to determine the concentration of gliclazide in plasma. The Photodiode array (PDA) detector allows for the determination of purity.
Subjects and The mobile phase used in this study was phosphate buffer with pH = 3.5 and acetonitrile (HPLC grade) at a ratio of 1: 1 was run isocraticaly through a C18 analytical column. The UV detection was done at 253 nm. Analytical run time was less than 10 min.
In the optimum conditions, the calibration curve in the area of ​50 to 400 ng/ml, linear with the equation y = 28.029x 212.74 and 0.998 = R2. Gliclazide was detected within 3.6 minutes by photodiode detector. The limit of detection (LOD) and the limit of quantitative (LOQ) in this method are calculated as 23 ng/ml and 71 ng/ml, respectively.
The evaluation of the accuracy and precision of method showed that this method has the ability to determine the amount of gliclazide in plasma, and the method is reliable and fast.