The comparison of N- acetylcysteine and Vitamin D on oxidative Liver toxicity in sub-acute paraquat poisoning

Paraquat is mainly used as a herbicide and its toxicity is through the increase of reactive oxygen species, in this study, we investigated the effect of N-acetyl cysteine and vitamin D on the oxidative toxicity of Liver tissue in sub-acute toxicity with paraquat.
In this experimental study, 36 male albino Wistar 8 weeks old were randomly divided into 6 groups: 1) control group ; 2, 3) control received Vit D 2 μg/kg/day or NAC 6.25 mg/kg/day Intraperitoneal injection (IP) ; 4) received paraquat 5 mg/kg/day (IP) ; 5 and 6) received paraquat treated with Vit D 2 μg/kg/day or NAC 6.25 mg/kg/day For 7 days. At the end of the study, serum and Liver tissue collected. Total antioxidant capacity (TAC), lipid peroxidation (LPO), total thiol groups (TTG), ALT and AST levels in Liver tissue were evaluated. Also liver histopathological evaluation was performed.
In paraquat-poisoned groups, liver tissue damage, LPO, ALT, AST were increased, and TAC, TTG decreased significantly in comparison with the healthy control group. In treated groups, there was a significant decrease in Liver injury, LPO and liver enzymes in comparison with paraquat group, and TAC and TTG levels significantly increased.
Vit D and NAC decreased oxidative toxic stress, and tissue damage in the Liver caused by paraquat poisoning.