Specialized Screening and Purification of Thalassopiria and Chromohalobacter Bacteria from Oil Sludge using Chemotaxi to Petroleum

Large amounts of waste from various stages in the process of refining crude oil are produced in the Persian Gulf in Iran. This waste is a viscous oil sludge containing high amounts of petroleum derived hydrocarbons. So oil sludge is environmental concerns and should be treated. Biological treatment (bioremediation) to deal with a wide range of organic pollutants and petroleum hydrocarbons is useful. In this study, the oil sludge has been obtained from the contaminated site from Bahregan area in the Persian Gulf of Iran. Some oil sludge containing bacterial consortium, Chemotaxis in synthetic Mineral semisolid agar medium was used for the isolation of alkane degrading bacteria from oil sludge biodegradation of octadecane, hexadecane, naphthalene, and anthracene were determined. Strains isolated in this study were identified by 16S rRNA gene sequencing as Thalassospira and Chromohalobacter. They are Gram negative and belonged to Rodospiracea which could hydrolyzed aromatic and linear oil compound.