Genetic diversity of date palm cultivars in Sistan and Baluchestan and Hormozgan provinces using microsatellite

Considering the Economic importance of date palm, in this study, 14 varieties of male and 26 female cultivars of date palm were evaluated using SSR primer. DNA of young leaves of date palm was extracted by CTAB method and it was propagated by 10 pairs of SSR primers. The propagated products were observed by 8% acrylamide gel and coloring with AgNO3. Using the software Gene Alex6.3 results of alleles and observed heterozygosity and polymorphism information content were calculated. Genetic relationships among cultivars were represented by a dendrogram based on the Nei’s Genetic similarity coefficient and Nearest joining method which was considered for cluster analysis. The results showed that polymorphism information content was calculated for all pairs of primers which varied from 63% for HQ542225 to 85% for HQ542208 (mean = 77%). The number of alleles in each individual locus varied in the range of 6-9 with the mean of 7.6. The 10 primers used a total of 76 alleles were detected primers and primers HQ542208 and HQ542224 with 9 alleles most HQ542225 6 DP169 and allele showed the lowest number of alleles. The highest genetic similarity was found between Almehtary and Khanizi and the lowest one between Abonarenja and other cultivars. According to the results of this study, a more comprehensive picture of the genetic structure of male and female date palm varieties has been obtained, which makes it possible to use them according to the case study of palm date cultivars.