Serological Detection of FMD Serotypes by New Prepared Innovative Recombinant Hepta-Epitopic Peptide

Foot-and-mouth disease (FMD) is a highly contagious and economically important disease that affects cloven-hoofed animals worldwide. In recent years, a series of outbreaks of FMD have occurred in many countries. Recombinant protein synthesis incorporating protective B- and T-cell epitopes are candidates for new safer and more effective (FMD) vaccines that have potential to provide protective immunity against diverse FMDV strains and to protect against future epidemics.

The aim of this study was to produce Recombinant Hepta-epitope peptide for detecting FMD O, A , Asia1 serotypes ,which can be used as a tool for diagnostic kits.

In the present study, we designed and produced a Recombinant Hepta-epitopic peptide from FMDV epitopes of the viral proteins VP1, VP2, VP3, 3C (seven epitopic regions, amino acid residues VP1 140-160, Vp1 200-213, VP2 68-75 ,VP2 179-198 ,VP3129-148 ,VP3 190-199 , 3C121-135).

It was shown that the mentioned recombinant peptide could recognize the serum collected from cattle infected with FMD serotype A and O.

Therefore we believe that this recombinant Hepta-epitopic peptide can be used for diagnostic serological assays. Furthermore this Recombinant peptide may be a potential candidate as an alternative vaccine against FMDV epidemic variants