Effect of Plant growth regulators on shoot induction of four thousand years old Abarkooh cypress

Abarkooh cypress (Cupressus sempervirens L. var. horizontalis(Mill) Gord) is one of the oldest cypresses in the world. Tissue culture is a method for saving and propagation of endangered and rare plants such as this cypress. This study aims at investigating the effects of different cytokinins and auxin plant growths regulators and coconut milk on micropropagation of plant. Experiments were performed as completely randomized design with six replicates. After sterilization for proliferation, explants were cultured in WPM medium culture by different treatments consisted of 1. Plant growth regulators (Kin, BA, TDZ and 2ip) at concentrations of (0, 0.1, 0.5, 1 and 2 mg/l) 2. Interaction effect of 0.1 mg/l cytokinins (Kin, BA, TDZ and 2ip) with IBA (0 and 0.01 mg/l) and 3. Coconut milk (0 and 50 ml) + 0.05 mg/l IBA. According to the results the number of shoots in culture media contained 1 mg/l BA and 0.1 and 1 mg/l 2ip increased. Addition of 0.01 mg/l IBA on culture media contained kin, TDZ and BA increased the number of shoots, but decreased the shoot length. Using coconut milk significantly increased the number of shoots and decreased their length. For root induction different media cultures of WPM, SH and LS with different hormonal treatments was used and only in WPM medium culture contained 2 mg/l kin root was obtained.