Cloning and expression of the NP gene of H9N2 avian influenza virus in Escherchia coli

Avian influenza (AI) is a serious infectious disease with world-wide significance causing considerable financial losses in poultry industry.Serodiagnosis based on detection of antibody to the nucleoprotein (NP) that is conserved among all influenza A virus, can be used to demonstrate immunity against all subtypes of influenza A virus. The aim of this study was to produce recombinant nucleoprotein (NP). For this purpose, the coding region of nucleoprotein (NP) gene of A/chicken/Iran/AH-1/06(H9N2) AI isolate was amplified by RTPCR and cloned into a prokaryotic expression vector (pMal-C2x) and transformed into E. coli BL21(DE3). The purified recombinant vector for the expression of recombinant MBP-NP was used.Target protein with a weight of about 97 kDa by SDS-PAGE and subsequent Western blotting was evaluated. Observationthe expressed protein with expected molecular weight in SDS-PAGE and positive reaction ofthis protein with bird ,serum infected avian influenza In western blot showed that the expression of NP gene in E.coli was successful.