Association of adiponectin rs17300539 gene polymorphism with a non-alcoholic fatty liver disease in an Iranian population

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disorder, resulting from exceeding fat cumulating in the liver .Adiponectin, a protein secreted from the adipose tissue, reduces liver inflammation. In this study, the relationship between adiponectin rs17300539 gene polymorphism and a non-alcoholic fatty liver disease was investigated. This case-control study was conducted on 80 subjects with NAFLD and 80 healthy subjects. The determination of polymorphism rs17300539 of adiponectin gene was performed by the PCR-RFLP method and electrophoresis technique. The plasma levels of adiponectin and insulin hormones were measured by an enzyme-linked immunosorbent assay (ELISA) kit. The results showed that there was no significant difference in allele frequencies between the two groups of the case and control (P>0.05). The body mass index (BMI) in genotype GA carriers was higher than that of genotype GG carriers (P<0.05). Moreover, the diastolic blood pressure in the male patients carrying the genotype GA was higher than that in the genotype GG carriers (P<0.05). In the female patients carrying the genotype GA, the AST and triglyceride levels were higher than the GG female carriers (P<0.05). It seems that the allele G can be beneficial in reducing the side-effects of the non-alcoholic fatty liver disease.