Survey of phytochemistry of Marrubium astracanicum Jacq. in Fars province

Marrubium astracanicum Jacq. is belonging to the Lamiaceae family. In this study, five populations of M. astracanicum (Derak, the west of Dasht Arjan, Bavanat-Simakan (Lakposhti), Ghorogh Abolmahdi (Pasargad) and Sadra areas) from Fars province were collected. After drying the plant samples, the essential oils (EOs) were extracted from the aerial parts by using the Clevenger Apparatus, and analyzed by Gas Chromatography (GC-FID) and Gas Chromatography/Mass Spectrometry (GC/MS). Methanolic extract was prepared by maceration method and analyzed by HPLC for polyphenolic compounds. EO yields were from 0.21 to 0.34% (w/w) based on dried material. The major constituents of the EOs were n-decane, n-dodecane, germacreneD, n-hexadecanoicacid, (E)-caryophyllene, bicyclogermacrene, phytol, n-tricosane, n-pentacosane, n-heneicosane and caryophyllene oxide. The 12 major compounds were analyzed by Minitab V.14 software. The findings of chemical compounds showed that despite the difference in height and climate, Sadra region was more similar to Derak and Lakposhti Bavanat. The standards of gallic acid, quercetin, catechin, caffeic acid chlorogenic acid were measured in the wavelengths of 280nm and and 320nm. The amount of polyphenols varied in different areas. Our results showed that the height and climate in each area caused the variation of polyphenolic compounds.