Investigation of the effect of phosphate solubilizing bacteria on yield and content of phosphorus of leaf and seed of canola

The phosphorus plays an important role in the growth and development of rapeseed. However, in a short period of time, two thirds of the phosphate fertilizer used is stabilized inaccessible in soil. Therefore, the only way to use phosphate accumulated in the lands is the use of phosphate biologic fertilizers. The present experiment was carried out in factorial in randomized complete blocks design with three replications in 2010 and 2011. Treatments including five bacteria of Pseudomonas (Pseudomonas fluorescence 1 (PSf1), Pseudomonas fluorescence 2 (PSf2), Pseudomonas putida 1 (PSp1), Pseudomonas putida 2 (PSp2) and not using bacteria (PS0)) and four levels of phosphorus fertilizer triple superphosphate (without fertilizer, 100, 150 and 200 kilogram per hectare) and two rapeseed cultivars, Hayola401 and SariGol. The results showed that the effect of different levels of phosphate solubilizing bacterial treatments on seed yield, biological yield, one thousand-seed weight, leaf and seed phosphorus content was significant at one percent level. The effect of different levels of phosphorus fertilizer on seed yield, biological yield, one thousand-seed weight, leaf and seed phosphorus content at one percent statistical level and on the number of pod per plant were significant at five percent level. The interaction of using phosphorus fertilizer and phosphate solubilizing bacteria on seed yield, leaf and seed phosphorus content was significant at one percent probability level. Comparison of averages showed that the use of phosphate solubilizing bacteria, especially Pseudomonas putida 2 (PSp2) can reduce the consumption of phosphorus fertilizer by 25 percent, thereby reducing production costs and environmental hazards.