Study of Pomegranate Hydromethanol Extract on Staphylococcus Aureus and Escherichia Coli by Microplate in Laboratory Conditions

Pomegranate skin contains large amounts of antibacterial and antioxidant compounds. Increasing antibiotic resistance in Escherichia coli bacteria as a major contributor to urinary tract infections and Staphylococcus  aureus is a cause of resistance to treatment-resistant infections, a serious need for alternative therapies or compounds that exhibit less resistance. The aim of this study was to investigate the antibacterial properties of Pomegranate extract and inhibit antibiotic resistance of the extract against pathogenic bacteria. After collecting fresh Pomegranate skin, they were dried in a place of sunlight and in shade for 48-72 hours and extraction was done by maceration, in order to determine the minimum level of exposure (Dilution) inhibitor was used from the Muller Hinton Broth and Brain heart Infusion Agar (BHI: Bullet Hit Indicator). The resulting turbidity was measured in a well solution (microdilution). The results of microdilution test showed that the growth inhibitory concentration (MIC: Minimum Inhibitory Concentration) was equal to dilution of 1.8 to 25 μL from Pomegranate extract for E. coli and Staphylococcus aureus bacteria. The growth inhibitory concentration (MIC) was equal to a dilution of 1.16 times 12.5 μL of Pomegranate extract. The extract extracted from Pomegranate skin has the most antimicrobial properties in comparison with other cases. This extract has the most antimicrobial effect on Staphylococcus aureus bacteria from gram-positive bacteria and against E. coli bacteria that is an indicator of fecal contamination. Finally, Staphylococcus aureus showed the highest susceptibility to this extract.