Identification of DNA markers related to phytochemical characteristics in Sumac (Rhus coriaria L.) fruits

Sumac (Rhus coriaria L.), recognized in Iran and other areas of the Middle East as a very popular flavoring spice, contains a wide range of medicinally active components including organic acids, phenolic acids, flavonoids, anthocyanins, hydrolyzable tannins and terpenoids. This plant species has suitable divergence in North West of Iran. In present research, samples of leaf and fruit of 15 sumac genotypes from each of 5 regions in north west Iran were conducted to fingerprinting using ISSR markers and also measuring Malic acid, Cumaric acid and Protocatechuic acid using LC-MS/MS. Inter population genetic diversity was higher that intra population variation for malic acid and Cumaric acid. Study of population structure of 75 sumac genotypes using 18 ISSR primers data including 132 ISSR markers and Structure software have divided them into two sub-populations. Results of association analysis revealed that markers including UBC867, UBC801 and UBC864 have significant association with genomic regions controlling Malic acid, Cumaric acid and Protocatechoice acid, respectively. Markers which identified in present study could be applied in sumac breeding programs.