Evaluation of Benzylisoquinoline Alkaloids (BIA) production in Papaver bracteatum cell suspension culture using plant growth regulators

Phytohormones and PGRs are influencing factors in the production of secondary metabolites. Papaverace family includes valuable alkaloids with commercial importance and Papaver bracteatum belongs to this family. Benzylisoquinoline alkaloids are a diverse group of more than 2500 specified natural compounds with medicinal effect which are found in 5 plant families including Papaveraceae. In vitro plant cell culture is a promising source for high rate secondary metabolites production. In this study, the effect of methyl jasmonate (MJ) and phloroglucinol (PG) in three different concentration, 0, 100 and 200 uM and mg/L, individually and in combination together, on the stimulation of thebaine and sanguinarine production in Papaver bracteatum cell suspension cultures was studied. Both elicitors significantly increased yields of thebaine and sanguinarine production compared to the control. Thebaine production 48 hours after elicitation with concentration of 200uM MJ and 100mg/L PG was about 68.63-fold and sanguinarine production at the same period with concentration of 200uM MJ and 200mg/L PG increased about 107.71-fold more than control. It seem that increases can due to synergic effects of used elicitors in this suspension cell culture. Adding MJ and PG, each one lonely, also increased yields of thebaine and sanguinarine. The results of this study showed that the use of PGRs can motivate biosynthetic cycle of the production and accumulation of morphinan alkaloids in plants and levels of this alkaloids can be eleveted comparing to control plants.