Endurance Training Can Control Diabetic-Induced Over Expression of Neurotrophin-4/5 mRNA In Sensory Roots of Sciatic Nerve

The aim of present study was to investigate effect of six weeks of endurance training on NT-4/5 expression at sensory roots of sciatic nerve in male rats with diabetic neuropathy. To do this, a number of 20 Wistar male rats (eight weeks of age) with average weight of 250±20g were used. Then rats were classified randomly to four 5-rats groups including diabetic exercised group, diabetic control group, healthy exercised group, and healthy control group. Two weeks after inducing the diabetes, endurance exercise protocol was executed moderately (50-55% of VO2max) for five days a week. Speed and time of treadmill exercise was increased gradually. The Quantitative Real time-PCR technique was used to measure the mRNA NT-4/5 expression. In addition, for determining the difference among the groups, the One-way ANOVA were applied. The findings resulted from the research revealed that diabetes significantly increases the expression of NT-4/5 gene at sensory roots of sciatic nerve (P≤0.001). Also 6 weeks of endurance training significantly increases expression of NT-4/5 gene at sensory roots of sciatic nerve of healthy rats (P≤0.05). However, 6 weeks of endurance training has no effect on expression of NT-4/5 gene at sensory roots of sciatic nerve of diabetic neuropathic male rats (P=0.938). However, NT-4/5 expression in diabetic exercised group was significantly lower in comparison with diabetic control group (P≤0.001). Therefore, diabetic neuropathy and endurance training alone can increase the expression of NT-4 gene at sensory roots of sciatic nerve, however, these effects decrease together.