Effect of 8 Weeks Aerobic Exercise Training with Chlorella Supplementation on Catalase and Superoxide Dismutase in the Heart of Diabetic Male Rats

The aim of the current study was to determine the effect of eight weeks aerobic training with chlorella supplementation on catalase and superoxide dismutase in the heart of diabetic male rats. Fifty Wistar rats (14-12 weeks old) with an average weight of 220 ± 10 g were kept in polycarbonate cages with a temperature of 20-22 ° C, relative humidity of 45 ± 10 ° C, and 12-12 hours of light / dark cycle, with free access to water and foods. After one week of familiarization with laboratory environment, they were randomly divided into 5 groups: (1) exercise, (2) supplementation, (3) exercise + supplementation, (4) diabetic control, and (5) non-diabetic control. The training program lasted for 8 weeks at speeds of 10-21 m/min and lasted 10-50 minutes. Diabetic induction was performed with a single intraperitoneal injection of Streptosucin (STZ) solution dissolved in a 0.1 M citrate buffer and 55 mg/kg body weight. Superoxide dismutase and catalase enzymes were measured by ELISA method. Variance analysis (2 × 2) was used for data analysis. All interventions (chlorella, exercise, and exercise plus supplementation) resulted in decreased blood glucose levels in diabetic rats. The results of the current study showed that after the intervention period, the amount of superoxide dismutase and catalase enzymes was significantly lower in all diabetic groups than non-diabetic healthy rats, and all three types of interventions prevented the diabetes-induced reduction in superoxide dismutase and catalase enzymes. However, although there was no difference between the exercise and supplementation groups, the combined group prevented more of the reduction in superoxide dismutase and catalase enzymes in camparison with exercise and supplementation separately. Therefore, it can be concluded that eight weeks of chlorella supplementation, aerobic training, and chlorella supplementation with aerobic training prevent the diabetes-induced reduction in superoxide dismutase and catalase enzymes in the heart tissue of diabetic male rats.