Effects of Endurance Training and Methanolic Otostegia Persica Extract on Betatrophin Serum Concentrations and Histopathological Changes of Streptozotocin Diabetic Rats

Betatrophin known as new therapeutic target of pancreases tissue in diabetes. The aim of this study was to investigate the effect of endurance training with methanolic Otostegia Persica extract on betatrophin serum concentration and histopathological changes in diabetic rats. Male Wistar rats (N=40) divided into 5 groups included healthy control (HC), diabetic control (DC), diabetic endurance training (DET), diabetic Otostegia Persica extract (DOP), and diabetic Otostegia Persica extract with endurance training (DOPET). After induction of diabetes, training groups performed 4 weeks endurance training and Otostegia Persica treatment group received 200mg/kg Otostegia Persica extract. Betatrophin concentration was assessed by ELIZA and pancreases tissue assessed by hematoxylin-eosin. Results showed that betatrophin serum concentrations was significantly increased in DOP (P=0.002) and in DOPET groups (P=0.01). Compared with DC groups, insulin resistance was significantly lower in DOPET (P=0.007). The results showed no significant differences in beta cell function in all groups (P=0.65). The mean of number of β-cell and size of pancreatic islets was significantly higher in DOPET group (P≤0.05). It is can be concluded that Otostegia persica extract was the main effect on serum betatrophin concentration, also endurance training with Otostegia persica extract was the main effect on histopathological changes in diabetic rats.