Investigation of Microbiota of Artemia franciscana Fed by Bacterial Probiotic Bacillus subtilis and Yeast Saccharomyces Cerevisiae under Laboratory Conditions

Artemia is one of the most important live foods in aquaculture. The aim of the current study was to investigate Microbiota of Artemia franciscana fed by bacterial probiotic Bacillus subtilis and yeast Saccharomyces cerevisiae under laboratory conditions. In this experimental research, 500 Artemia nauplius were transported to 1 liter Zug with salinity of 70ppt and fed daily by Dunaliella tertiolecta and wheat bran. The present study was carried out in 4 treatments, including control treatment (no additive probiotics), probiotic treatment of Saccharomyces cerevisiae, probiotic treatment of Bacillus subtilis, and equal combination of yeast and bacteria. Statistical tests including Duncan's new multiple range test and one-way analysis of variance were used. SPSS 21 and Excel 2013 software were also used. Findings: Bacterial probiotic treatment showed the highest colony count compared with other treatments (p<0.05). Treatment containing equal combination of yeast and bacteria had the highest colony after probiotic bacterial treatment (p<0.05). The highest number of Bacillus colonies in the body was obtained in probiotic bacterial treatment and combination of yeast and bacteria, respectively, which was significantly higher than control and yeast treatments (p<0.05). Based on the total number of Bacillus colonies, probiotic bacterial treatment showed a significant difference with other treatments and the lowest number of colony was related to probiotic yeast treatment (p<0.05). Compared with other treatments, the highest number of lactic acidic bacteria is in bacterial treatment. These probiotics can well place on the surface as well as inside the body of Artemia. Therefore, using Bacillus subtilis alone or with Saccharomyces cerevisiae can have a positive effect on the bacterial count of Artemia