Evaluating the viability of adipose tissue-derived mesenchymal stem cells cultured on decellularized sciatic nerve scaffold of STZ-induced hyperglycemic male Wistar rats treated by benfotiamine

The purpose of this study was to investigate the effects of benfotiamine treatment of hyperglycemic rats on viability of adipose tissue- derived mesenchymal stem cells on sciatic nerve acellular scaffolds. Material and method: After induction of hyperglycemia (STZ) and treatment with benfotiamine segments for 4 and 8 weeks from the middle part of the sciatic nerves were decellularized using Sandell method and seeded with mesenchymal stem cells derived from adipose tissue. after 8 days of culture cells viability in the culture medium was evaluated by MTT assay and adhesion of the cells to the scaffold was examined by scanning electron microscopy (SEM). In comparison with intact group, cell viability of untreated hyperglycemic rats was significantly decreased while, there was no significant difference between benfotiamine treated group and intact. Results of electron microscopy confirmed adhesion of the cells on the scaffolds. In hyperglycemic condition it is likely that glycosylation of ECM constituents and the production of AGEs decrease the inducible effects of ECM on the level of cell viability and probably cell adhesion to the scaffolds. It seems that benfotiamine treatment of hyperglycemic rats by reduction of glycation and AGEs production may prevent the structural changes of the ECM.