Investigation of POU5F1 and NANOG gene expression in colon cancer cell line (Caco-2) treated by dendrosomal nano-curcumin

Colon cancer is the fourth most common cancer. With concern to high prevalence and mortality of colon cancer, it is obvious the importance of drug related study, such as effective component in prevention, therapy and recurrence of the cancer. Curcumin is natural yellow pigment isolated from rhizome of turmeric. Curcumin is a new candidate for anticancer treatment, but its low bioavailability and water solubility represent the main disadvantages of its use. In this study, the role of dendrosomal nano carrier assessed in increment of curcumin effects in colon cancer cell line (caco-2). For this reason, IC50 was determined by MTT assay. Also, the effects of dendrosomal nano-curcumin recognized with gene expression study of POU5F1 and NANOG by Real Time PCR. Our finding showed that dendrosomal nano-curcumin decrease cell growth depends on time and concentration, moreover, it significantly declines POU5F1 and NANOG gene expression in colon cancer cell line (caco-2). Overall, the results showed that dendrosomal nano-cucomin could be effective in therapy of colon cancer by reducing the expression of genes involved in unlimited cell proliferation.