Identification of DR75-RS11090 Gene Encoding GGDEF Domains, and its Role in Enterococcus faecalis

Enterococcus faecalis is considered the primary pathogenic bacteria in failed root canal therapy due to its excellent biofilm formation ability. The second messenger cyclic dimeric (3’→5’) GMP (c-di-GMP) is involved in bacterial physiological processes, including biofilm formation. C-di-GMP is formed by diguanylate cyclases with the GGDEF domain. The current study aimed at investigating the function of DR75-RS11090 in E. faecalis. In the current experimental study, gene encoding GGDEF domains in E. faecalis were analyzed through National Center for Biotechnology Information (NCBI) database. Enterococcus faecalis strains were cultured in brain-heart-infusion (BHI) broth at 37°C. Growth curves of E. faecalis were measured in an automatic microplate reader. Biofilms were fixed with 2% glutaraldehyde and scanned under the transmission electron microscopy. Acid and water-insoluble polysaccharide productions were also detected by the precise digital acidometer and anthrone method. It was found that DR75-RS11090 gene was predicted to encode GGDEF domain in E. faecalis. The current study observed promoting effects of DR75-RS11090 on E. faecalis biofilm formation, growth, acid production, and water insoluble polysaccharide production. The current study findings improved the understanding of c-di-GMP in E. faecalis and presented a primary study on DR75-RS11090 promoting mechanism.