The effect of single bout of continuous and high intensity interval exercise on VEGF levels in adipose tissue in obese male wistar rats

Continuous and interval exercises cause losing weight and changing in adipose tissue. The goal of this study is to investigate the effect of single bout of continues (CON) and high intensity interval exercise (HIIE) on the VEGF levels in adipose tissue of obese male Wistar rats. After high fat diet for eight weeks, 30 rats with BMI: 0.68 g/cm2 were chosen. The exercise program in HIIE group was 5sets with 100% vVo2max and 4 sets with 30% vVo2max. The intensity in CON group was 65% vVo2max. Both groups started their exercise after 3 minutes of warming up. The control group didn’t take part in any exercises. Immediately after exercise animals were anesthetized and then visceral and subcutaneous adipose tissues were removed. Measuring the VEGF levels was performed with western blotting method. we found that the single bout continues exercise has increased 39% VEGF levels (P= 0.05), while single bout HIIE has decreased (P=0.012) in visceral adipose tissue. VEGF levels in subcutaneous weren’t measurable because of low levels of VEGF or mistake in removing tissue. Continues exercise was effective in increasing of VEGF. Decreasing of VEGF levels was due to hypo perfusion in HIIE group during exercise. Because of dysfunction of angiogenic capacity in adipose tissue of obese people to stimulate more adaption and accurate diagnosis, recommended that changes of VEGF levels should measure in different times after exercise.