Investigation of Establishment Possibility of Royal Jelly-induced Polycystic Ovary Syndrome Disorder Model in Rat

Human studies have great challenges in understanding the pathogenesis of polycystic ovary syndrome due to ethical and practical limitations and animal models have high value in the study of this syndrome. Regarding estrogenic properties of royal jelly, the aim of this study was to investigation of establishment possibility of royal jelly-induced polycystic ovary syndrome disorder model in rat. In this experimental study,40 adult virgin female rats strain weighing 180±20grwere divided in 5 groups of 8. control group without treatment, sham group received normal saline as treatment solvent and 3 experimental groups respectively intraperitoneally injection received 250, 500 and 1000mg/kgBW of royal jelly for 21 days.At the end of the treatment period the blood samples were taken from all groups, serum concentration of sex hormones were measured and the follicles were counted by isolating the ovaries and preparing the tissue sections. The data were analyzed using one-way ANOVA. The results of this study showed that the serum concentration estradiolhormone and the number of primordial follicles in the 250mg/kgBW recipient group were significantly increase than control group (p<0.05); Also ovarian cysts were not observed in histopathologic examination of ovarian tissue. Royal jelly increases serum concentration estradiol hormone and the number of primordial follicles at a minimal dosage but with increasing does not changes in serum concentration of sex hormones also tissue and ovarian function.