Synthesis of Copper nanoparticles using aqueous extract of Postia puberula flora and evaluation of their antimicrobial activity.

The development of nanotechnology is making the intrest of researchers towards synthsis of nanoparticles for the bio application. In the present study, copper nanoparticles (CuNPs) have been synthesized by the reduction of copper sulfate using aqueous extract of Postia puberula Flora.The green synthesized CuNPs were characterized using UV-Vis absorption spectroscopy, Scanning Electron Microscopy (SEM) and Enerrgy Dispersive X-ray analysis(EDX) The antimicrobial activity of CuNPs was determined by disc diffusion and broth microdilution methods against some selected species of bacteria. After addition of flower extract, the change in colour of Copper Sulfate solution was noted from light blue to sea green. The UV-Vis absorption spectra confirmed the formation of the CuNPs with the characteristic peak 575 nm. . XRD and SEM analysis of copper nanoparticles indicated that they exist in amorphous in nature and with size ranged from14 to 39 nm. Fruit extract acts as both reducing and capping agent. The synthesized Cu nanoparticles exhibited activity against of both gram positive and gram negative ba cteria.