The effect of ultrasound method on the extraction of chlorophyll from mulberry leaves

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
The aim of this research was optimizing of the chlorophyll extraction by ultrasound method. A Central Composite Design with 6 repeats at the central point was employed to optimize the effect of the ultrasound power (10-100%), solvent to solid ratio (5-10 ml/g), temperature (30-70 °C ) and time (2-10 min) on the total chlorophyll and a* value. Results of optimization showed that a quadratic model with R2=0.922 was significant. The solvent to solid ratio was the most important parameter which positively affected the yield, although increasing the temperature of extraction significantly reduced the yield. Enhancing the ultrasound power considerably increased the yield of chlorophyll. The maximum yield of chlorophyll was obtained under power of 100%, temperature of 30.2 °C, time of 9.83 min and solvent to solid ratio of 9.99 (ml/g) which was 680.5 mg/100 g d.m. The comparison of conventional and ultrasound methods showed that the yield of chlorophyll extracted by conventional method (460.5 mg/100 g d.m.) was significantly lower than the amount (680.5 mg/100 g d.m.) extracted by ultrasound method. Therefore, the ultrasound method is an efficient method for extraction of chlorophyll.
Language:
Persian
Published:
Journal of Innovative Food Technologies, Volume:4 Issue: 4, 2017
Pages:
67 to 76
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