Study of Quantity and Diffusion of Nisin Used in Corn Zein Film to Depopulate Micrococcus Luteus

Nisin is a bacteriocin that loses its activity due to binding to the protein, fat and other in the food systems. By preparing a film based on the biopolymer can improve its performance. The objective of this research was to investigate the antimicrobial activity and quantity diffusion of different concentrations of nisin (100, 200 and 300 mg) from 4 percent corn zein film in phosphate buffer and evaluation of antimicrobial activity and quantity diffusion of nisin from different concentrations of corn zein films (2, 4, 6, 8 and 10) in the phosphate buffer. The antimicrobial activity of nisin diffusion from corn zein films was performed with evaluation of growth inhibition of Micrococcus luteus in culture media and its quantity was evaluated with high-performance liquid chromatography method. In the final stage modeling of nisin release kinetics performed via empirical Weibull model. According to results the antimicrobial activity of nisin was confirmed by formation of growth inhibition halo in agar. Quantity diffusion of nisin from 4 percent corn zein film significantly increased with increasing concentration of nisin. With increasing the concentrations of corn zein from 4 to 10% (W/V) the nisin diffusion from corn zein film 10%, four times decreased which related to increase kink in film and slower release of nisin. Kinetic modeling diffusion from films showed similar behavior Fick with excellent prediction (R2 >0.95). Therefore by using the corn zein edible coating containing nicin antimicrobial composition can increase the shelf life and quality of food.