An introduction to RNA sequencing

High-throughput sequencing technologies are now in common use in biology. RNA seq is one of the next generation sequencing that applies for transcriptome profiling. This method with deep sequencing of complementary DNA allows researchers to measure RNA abundance on a genome-wide scale and alter our view of the extent and complexity of eukaryotic transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts than other methods. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection and detection of alternative splicing events and isoforms. Ongoing developments of this technology get possible of direct RNA sequencing and profiling low-quantity RNA samples. In this review article will be introduced RNA-seq technology and implied some developments that overcome limitation of other methods.