Generation of induced pluripotent stem cells (IPSCs) (A glancing review about methods and techniques)

Cell therapy is one of the most principle and promising science in relation to treatment of many diseases. By attention to unique capability of stem cells, these cells can be for treatment as a valuable source in science and medical researches. However, there are a lot of problems in relation with use of embryonic stem cells, which has limited their use. Thus, requirement to find an alternative technique to produce these cells, is one of the most important concerns of scientists to improve and use of this science. Induced pluripotent stem cells, is one of these technologies. Different methods which have the ability of altering gene expression profile and cell proteins, cause to change cell morphology and function in reverse way to undifferentiated cells and generation of stem cells, are developing. These methods include nuclear transfer, use of cell extract and synthetic molecules, forced expression of defined genes and change in cytoplasmic levels. Nowadays, there are development in nonintegration ways, but about increasing efficiency of these methods, many researches are ongoing. In this article techniques, advantages and ahead opportunities in generation of ips cells are examined.