The association study of rs3024998 and rs3025000 polymorphisms in VEGF gene with the risk of recurrent pregnancy loss

Recurrent pregnancy loss (RPL) is defined as the occurrence of two or more consecutive pregnancy loss prior to 20th week of gestation. There are several leading causes of RPL including uterine anatomical defects, genetic factors, infectious, immunological, environmental and blood dyscariasis. However, despite in a large number of cases no causes have been identified, therefore are introduced as idiopathic. Gene polymorphisms may effect on the incidence of abortion. One of these genes is the vascular endothelial growth factor (VEGF). Present study was aimed to investigate the association of VEGF gene polymorphisms with recurrent spontaneous abortion of the fetus. We conducted a case–control study of 200 women: 100 patients with at least two unexplained consecutive pregnancy losses and 100 healthy controls with at least one live birth and no history of pregnancy loss. Patients with recurrent pregnancy losses due to anatomic, hormonal, chromosomal, infectious, autoimmune, or thrombotic causes were excluded from the study group. Genomic DNA was extracted from peripheral blood lymphocytes. Rs3024998 and rs699947 polymorphisms of VEGF gene were studied using PCR-RFLP technique. Respectively, MaeI and BglII restriction enzymes were used for digestion. Digestion products were visualized by polyacrylamide gel (12%PAGE) electrophoresis. Distribution analysis for homozygous and heterozygous genotypes in the two studied groups was performed by using SPSS ver.18.Genetic differences between case and control groups were calculated by using the chi-square test. Significant difference (P<0.05) was identified between the frequency of allelic status of rs3024998 and rs3025000in two studied groups.