The determination of optimal condition for micro propagation of Cratagus aronia under in vitro culture

Crataegus Sp. (Rosaceae) has medicinal, ornamental and commercial utilizations but due to interspecific hybrization, apomixis and polyploidy, it has become one of the most difficult type of trees for botanists to propagate. To overcome this and to promote the process of controlled and mass production new technologies especially tissue culture can be used. Hence, this study was conducted for in vitro optimization of micropropagation of Cataegus aronia which includes shoot proliferation and rooting. Sampling was performed during spring. The samples were then disinfected and the explants were cultured on MS medium for initial establishment. The MS media was supplemented with concentrations of Indole acetic acid, Indole butric acid, Benzylaminopurine and Thidiazuranfor shoot proliferations and 1/2 MS or MS medium with concentrations of Indole butric acid and Naphthaleneacetic acid was used for rooting experiments. The results indicate that the best proliferation occurred on 3 mg/l Benzylaminopurine + 1 mg/l Indole butric acid and the 1/2 MS medium containing 3 mg/l Indole butric acid resulted in the highest percentage of rooting.