The Effect of Endurance Training on Expression of miR-196a , miR-133a and UCP-1, in Subcutaneous White Adipose Tissue of male Wistar Rats

The aim of current study was to investigate the effect of endurance training on expression of miR-196a and miR-133a and UCP-1 in subcutaneous white adipose tissue (WAT). Sixteen wistar rats were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training
on treadmill for eight weeks. The gene expression of target genes was measured by Real Time–PCR method. Data showed that gene expression of UCP1 was significantly higher in trained group than control (P<0.05). However, the expression of miR-196a and miR-133a were not significantly different
between trained and control groups (P>0.05). The results of this study showed that the endurance training despite the increase in the UCP1 expression, did not change the expression of miR-196a and miR-133a in subcutaneous WAT, indicating that the increase of UCP1 expression occurred by the endurance training did not correspond to change of miR-196a and miR-133a expression induced by the endurance training.