Assessment of relative gene expression of wdhn5 in wild and agronomy wheat genotypes under drought stress conditions

In order to examine the role of this regulatory factor and its gene expression pattern during stress in wheat plant as well as the physiological characteristics and expression of wdhn5 gene under drought stress as a  factorial experiment was carried out in completely randomized design in pot with three replications on  The wheat gemoyypes including two hexaploide cultivars (Bolani, Sistan) , two tetraploide cultivars(, Behrang, Shabrang) and wild (Urartu, Tauschii, Speltoides) were as the first factor and irrigation treatment with three levels consisted of normal (90% of field  capacity), mild stress (60% of field  capacity) and severe stress (40% of field  capacity) was as the second factor. Analysis of variance for physiological characteristics showed that the effect of water stress on all traits were significant, and the sensitive cultivar Speltoides had significantly lower dry mater production, water use efficiency and relative water content than the other cultivars. The result of the quantitave expression of wdhn5 gene expression by  real time PCR showed that expression of this gene in Tauschii and Bolani tolerant cultivars in 40% of field  capacity increased significantly which could indicte the importance of this gene in drought stress conditions. Based on these results, Bolani cultivar and Tauschii wild cultivar are relatively tolerant to drought and Splotheids is sensative to drought.