Association of the -308G/A Polymorphism of TNF-α Gene with Some Semen and Sperm Parameters in Men with Infertility
Background :
The tumor necrosis factor alpha (TNF-α) gene is a cytokine involved in systemic inflammation. Results of the association of its polymorphisms with infertility in men are controversial.
Objective :
The aim of this study was to evaluate the association of -308G/A polymorphism in TNF-α gene with different parameters of semen and sperm in infertile men.
Methods:
Participants were 210 men with infertility and 120 controls. The polymorphism evaluation was performed by sequencing method. In order to assess oxidative stress and apoptosis in men with -308G/A polymorphism, malondialdehyde and caspase-9 values were measured. The sperm DNA damage and sex chromosomes aneuploidy were also measured in these patients.
Findings:
A significant higher frequency of A allele of -308G/A polymorphism was observed in men with oligoasthenospermia (P=0.01). In semen samples of the men with AA genotype, there was significantly high level of malondialdehyde and caspase-9 (P<0.0001). We also observed higher frequency of sperm DNA damage (P=0.0001) and sex chromosome disomy type XY (P=0.0001) in sperm samples of men with AA genotype of -308G/A polymorphism. Furthermore, there was a significant correlation between sex chromosome aneuploidy and sperm DNA damage in sperms of men with this polymorphism (R=0.72, P=0.048).
Conclusion:
-308G/A polymorphism of TNF-α gene has association with decreased sperm parameters, increased oxidative stress, higher sperm DNA damage and sex chromosome aneuploidy which can affect infertility.
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