Fabrication of Ethanol Detection Electrochemical Biosensor Using Modified Platinum Electrode

In this research, alcohol oxidase was immobilized at platinum electrode surface using chitosan to design an accurate and sensitive electrochemical biosensor for detection and measurement of ethanol in liquid phase. Amperometric studies were carried out in 0.1 M phosphate buffer solution containing ethanol. According to the obtained results, the optimal conditions for biosensor operation were acquired at the temperature of 35 °C and pH 8. In order to improve the biosensor performance, gold nanoparticles were used in its construction and the comparison of obtained results from designed biosensor before and after adding these nanoparticles showed four-fold enhancement in current intensity produced by ethanol oxidation. The linear range of the gold nanoparticles modified biosensor, its Michaelis-Menten constant value, and its sensitivity were calculated to be 0.016-2.66 mM, 1 mM, and 2 µA/ mM cm2 respectively.