Comparison of anti-inflammatory effects of dexamethasone and thiamine on Wallerian degeneration after sciatic nerve transection in rat
The aim of this study was to investigate the apoptotic effect of the alcoholic extract of black seed on HL-60 cancer cells.
In this study, concentrations of 350, 650, 950 and 1250 µgml-1 of Nigella sativa alcoholic extract were added to HL-60 cancer cells for 24 h. MTT assay was applied to find out the portion of living cells. Likewise, PI staining for flow cytometry was performed to determine whether black seed extract inhibits the proliferation of HL-60 cells by modulating cell cycle progression and apoptosis, then AO/EB staining of HL-60 cells was performed to detect apoptosis and necrosis patterns.
MTT assay showed that in comparison with the control group, alcoholic extract of black seed killed 50% of HL-60 cell in the concentration of 650 µgml -1and based on the concentration, more cell death was caused at the higher concentration. Also, a substantial increase in G0/G1 phase cells was observed from 15. 2% in the control group, up to 51. 65% in the group was treated with 650 µgml -1 It was also found that the concentration of 650 µgml -1 induced apoptosis in HL-60 cells, while at higher concentrations of 950 and 1250 µgml -1 caused HL-60 cells necrosis compared to the control group.
Black seed , Apoptotic , HL-60 cells , flow cytometry , AO , EB staining
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
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