Study of genetic diversity of wheat bacterial blight (Pseudomonas syringae pv. Syringae) using ISSR molecule marker in Iranian native wheat cultivars

Bread wheat is the most important crop in the world whose yield is affected by several diseases including bacterial leaf blight. The most appropriate method for managing this disease is using resistant cultivars. DNA markers are highly effective in plant grouping in terms of resistance to diseases, and among these markers, the ISSR marker is recommended because of the do not need for prior information from the DNA sequencing and its easy and fast implementation. In this research, genetic variation of 27 Iranian indigenous wheat cultivars with respect to resistance to bacterial blight was investigated using 12 ISSR primers, which produced 170 evaluable bands, of which 156 were polymorphic bands. The highest percentage of polymorphic bands was belonged to F7, F8 and F10 primers, and the lowest of it was related to F9 primer. The highest content of the polymorphism information content (PIC) was belonged to the F10 and F5 primers and the smallest value belonged to the F7 primer. Cluster analysis classified these cultivars into four groups: Ghods, Omid and Atrak cultivars in the cluster of resistant group and other cultivars in sensitive and very sensitive group. The high polymorphism obtained in this study can be attributed to the high efficiency of the ISSR marker in evaluating the genetic diversity of wheat cultivars in terms of resistance to various diseases..