Ethnopharmacology, Phytochemical and Antioxidant investigation of some of traditional high consumption plants for treatment of diabetes in the rural mountainous rangelands of Chaharbagh

The present research was done in Chaharbagh rangelands of Golestan province due to phytochemical investigation of the most important anti diabetic native medicinal plants. Nine medicinal plants used by local people for diabetes treatment were consisting of Nepeta pungens L., Hypericum perforatum, Juniperus communis L., Artemisia annua L., Tanacetum parthenium, Urtica dioica L., Cichorium intybus L., Cuscuta reflexa L. and Perovskia abrotanoides Karel. After sampling plant parts containing Pharmaceutical ingredients consisting of aerial parts or root parts, they were cleaned and dried in the shade. Extraction of methanol was done. The amount of total phenol and flavonoids were measured with Spectrophotometry method and the level of antioxidant activity was measured with DPPH method. DCA ordination was done in order to studying relation between anti-diabetic medicinal plants and their quantity of effective ingredients. Results from phytochemical experiments showed that flowering part Hypericum perforatum, roots of Nepeta pungens L. and roots of Tanacetum parthenium with the amounts of 259.64, 231.246 and 223.47 mg GAE/g in plant dry matter had the most amount of total phenol. Artemisia annua L. leaves, flowering part of Nepeta pungens L. and roots of Urtica dioica L. with the amounts of 253.37, 237.89 and 216.43 mg QUE/g had the most flavonoids. The best antioxidant activity of the extracts with greatest DPPH inhibitory power was belonging to fruits of Juniperus communis L. aerial parts of Cuscuta reflexa L. and flowering parts of Perovskia‎ abrotanoides Karel. with amounts of 93.93, 81.58 and 72.24. The ordination analysis had the same results.